The duplexes were stored at −20°C until use. HEK 293, KB-3-1, and K562 cells were purchased from the Institute of Cytology RAS (St. Petersburg, Russia). B16 cells were kindly provided by the National ...

HLA-null human K562 cells were transduced with these alleles, expanded, and enriched for HLA expression by flow cytometry to produce single HLA allele lines. To induce isoLG adduct presentation, cells ...