Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
1 week ago
genome.gov
SOLVED: PCR Optimization and Lab CalculationsExerciseThe success of a PCR reaction depends on many factors: the concentration of the reagents used in the reaction, the temperatures used in the thermocycler, the quality of the DNA, the primer design, and others. For a typical 50 μL PCR reaction, the following parameters are used as a guide:Reagent Typical final concentration rangeDNA template 1 ng – 50 ng of plasmid DNA 5 ng – 50 ng of genomi
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